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Image Search Results
Journal: Oxidative Medicine and Cellular Longevity
Article Title: Simvastatin Treatment Upregulates HO-1 in Patients with Abdominal Aortic Aneurysm but Independently of Nrf2
doi: 10.1155/2018/2028936
Figure Lengend Snippet: Simvastatin treatment increases HO-1 protein, but not mRNA level in AAA. (a) Protein level of HO-1 (measured with ELISA) in aneurysm wall of nonstatin and simvastatin-treated patients. (b) Changes in total glutathione and the ratio of GSH/GSSG in aortic aneurysm wall. (c) Immunofluorescent staining of HO-1 in ECs and SMCs in aortic aneurysm wall. HO-1, heme oxygenase 1; Nrf2, nuclear factor E2-related factor-2; vWF, von Willebrand factor (EC marker); SMV, myosin smooth muscle heavy chain (SMC marker). (d) Nrf2 level in AAA tissue. Densitometry data and representative blot. (e) mRNA levels of Nrf2-dependent genes: NQO 1, GCLM , and HMOX1 in AAA wall. (f) Localization of Nrf2 in AAA wall. The brown color represents positive staining (IHC). Vasa vasorum is indicated by asterisks ( ∗ ). Inflammatory infiltration indicated by hash (#) was stronger in the nonstatin patient. (g) Illustrates the distribution of Nrf2 and HO-1 staining (IF) within AAA tissue. Average data from panels a, b, d, and e represents mean ± SE.
Article Snippet: HO-1 protein concentration was determined in tissue lysates using
Techniques: Enzyme-linked Immunosorbent Assay, Staining, Marker